Journal: Cancer Management and Research
Article Title: Identification of key genes and specific pathways potentially involved in androgen-independent, mitoxantrone-resistant prostate cancer
doi: 10.2147/CMAR.S179467
Figure Lengend Snippet: qRT-PCR analysis of DEGs identified in the microarray. Notes: Comparison of DEGs between PCa xenografts from castrated mice and parental cell lines. ( A ) CWR22R vs CWR22. ( B ) VCaPR vs VCaP. Expression data are represented by a log ratio calculated by comparing ΔCq from the xenograft with ΔCq from the parent cells. ΔCq was calculated as the difference between Cq of the targeted genes and Cq of the endogenous control gene, β-actin. Using this method, 16/18 (89%) genes pulled from the microarray analysis were confirmed to be differentially expressed by qRT-PCR. Abbreviations: DEGs, differentially expressed genes; qRT-PCR, quantitative reverse transcription PCR; PCa, prostate cancer.
Article Snippet: Gene expression profiles of MTX-treatment xenografts and their respective parental cell lines were performed using an Agilent whole human genome oligonucleotide microarray and analyzed using Ingenuity Pathway Analysis software.
Techniques: Quantitative RT-PCR, Microarray, Expressing